Abstract
A novel environmentally sensitive fluorescent (ESF) purine nucleoside, (cna)A, was synthesized and its photophysical properties were investigated. The base-modified fluorescent nucleoside (cna)A exhibited remarkable solvatochromicity and environmentally sensitive dual fluorescence. By using (cna)A, we have developed highly thymine (T) selective fluorescent DNA probes that can sense the corresponding T in a target DNA/RNA sequence, as measured by a distinct change in emission wavelength.
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