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Abstract
An electrochemical microbiosensor involving NAD-dependent dehydrogenase has been designed and developed using silicon technology. The sensor is based on a weakly polarized two-gold microelectrode system without any reference electrode. The process allowed mass production of miniaturized devices. An enzymatic solution including diaphorase and NAD-dependent dehydrogenase was confined in the microstructure by means of a semi-permeable membrane. The biocompatibility of the various materials was verified and the gold electrodes were electrochemically characterized. The assay of D-lactic acid was taken as an example using D-lactate dehydrogenase. The sensitivity and detection limit of the microbiosensor were 8 μA mM–1 cm–2 and 0.01 mM respectively. Its lifetime was 3 weeks.
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