Design of a peptide for immunodetection of IgA antigliadin antibody for the purpose of screening of celiac disease.

Rajasri Bhattacharyya,Dibyajyoti Banerjee,Neeru Sharma

doi:10.6026/97320630008087

Abstract

Celiac disease (CD) is gluten induced enteropathy which requires jejunal biopsy for diagnosis. To select the patients for endoscopoic procedure some serologic tests are popular in clinical practice for screening of CD. Although gliadin is one of the key immuno activator of the disease; serological screening by immuno-detection of gliadin is not recommended. In this context we have designed a peptide using tools of computational biology keeping molecular pathogenesis of the disease into consideration such that antigliadin antibody detection based sensitive and specific cost effective tool for screening of celiac disease can be developed. The designed peptide QPFPEP interacts in a stable manner with dimeric immunoglobin A1 molecule and its parent peptide QPFPQP are sequentially present in maximum number of gliadin epitopes. This hexapeptide is predicted to interact with dimeric IgA1, which increases in the biofluids of the CD patients. CD - Celiac disease, TT - Tissue transglutamase, IgA - Immunoglobulin A, AGA - antigliadin antibody, Immunoglobulin G - IgG.

Highlights

The genetic code is the sequence of nucleotides in DNA or RNA that determines specific amino acid sequence in synthesis of proteins
It can be inferred that high level of heterogeneity is seen within the genes of various functions in Chromohalobacter salexigens
Though codon usage of C. salexigens is largely determined by compositional constraints, translational selection is operating in shaping the codon usage variation among the genes

Summary

Introduction

The genetic code is the sequence of nucleotides in DNA or RNA that determines specific amino acid sequence in synthesis of proteins. It employs 64 codons, which can be grouped into 20 disjoint families, one family for each of the standard amino acid, and 21st family for translation termination signal. According to the number of synonymous codons related to each amino acid, for a gene using the universal code, there are two amino acids with one codon choice, nine with two, one with three, five with four, and three with six. It has been reported that there is significant variation of codon usage bias among different genes within the same organism [3,4]

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Conclusion