Abstract
This paper analyzed the degradation pathways of petroleum hydrocarbon degradation bacteria, screened the main degradation pathways, and found the petroleum hydrocarbon degradation enzymes corresponding to each step of the degradation pathway. Through the Copeland method, the best inoculation program of petroleum hydrocarbon degradation bacteria in a polluted site was selected as follows: single oxygenation path was dominated by Streptomyces avermitilis, hydroxylation path was dominated by Methylosinus trichosporium OB3b, secondary oxygenation path was dominated by Pseudomonas aeruginosa, secondary hydroxylation path was dominated by Methylococcus capsulatus, double oxygenation path was dominated by Acinetobacter baylyi ADP1, hydrolysis path was dominated by Rhodococcus erythropolis, and CoA path was dominated by Geobacter metallireducens GS-15 to repair petroleum hydrocarbon contaminated sites. The Copeland method score for this solution is 22, which is the highest among the 375 solutions designed in this paper, indicating that it has the best degradation effect. Meanwhile, we verified its effect by the Cdocker method, and the Cdocker energy of this solution is −285.811 kcal/mol, which has the highest absolute value. Among the inoculation programs of the top 13 petroleum hydrocarbon degradation bacteria, the effect of the best inoculation program of petroleum hydrocarbon degradation bacteria was 18% higher than that of the 13th group, verifying that this solution has the best overall degradation effect. The inoculation program of petroleum hydrocarbon degradation bacteria designed in this paper considered the main pathways of petroleum hydrocarbon pollutant degradation, especially highlighting the degradability of petroleum hydrocarbon intermediate degradation products, and enriching the theoretical program of microbial remediation of petroleum hydrocarbon contaminated sites.
Highlights
Petroleum hydrocarbon pollutants are classified as priority environmental pollutants [2], which primarily exist in the form of n-alkanes, branched-chain alkanes, cycloalkanes, and aromatic hydrocarbons in the environment [3,4]
This paper presents the results of the analysis of the degradation pathways of petroleum hydrocarbon pollutants in the presence of petroleum degradation bacteria
As the degradation pathway of the petroleum hydrocarbon pollutants is complex, we studied the main steps in the degradation pathway
Summary
The rapid development of the petroleum industry and its products, and the increase in the use of petroleum fossil fuels have led to serious environmental pollution. The pollution can be largely attributed to petroleum hydrocarbon pollutants [1]. Petroleum hydrocarbon pollutants are classified as priority environmental pollutants [2], which primarily exist in the form of n-alkanes, branched-chain alkanes, cycloalkanes, and aromatic hydrocarbons in the environment [3,4]. Most of them can exist stably in the environment for a long time and are not degraded. Crude oil leakage is one of the fundamental causes of petroleum hydrocarbon pollution, which has caused great harm to the soil ecosystem [5,6,7]
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