Abstract
A complete analysis of a novel method to evaluate the rotational diffusion time of fluorescent molecules is presented. It is based on the study of the total fluorescence produced after excitation with two consecutive short laser pulses. Expressions for the total fluorescence energy emitted in this case are derived, which would allow the evaluation of the rotational diffusion time. The main advantages of the proposed method are its simple setup, a time resolution only limited by the exciting laser pulsewidth, and the fact that it does not require fast time response detectors.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
