Design, fabrication, evaluation, and in vitro study of green biomaterial and antibacterial polymeric biofilms of polyvinyl alcohol/tannic acid/CuO/ SiO2

Abstract

In this study, a three-component biofilm for rapid wound dressing consisting of polyvinyl alcohol (PVA)/tannic acid (TA)/with CuO/SiO2 with different percentages (0, 5, 10, and 15 wt% NPs) is evaluated. In addition to controlling bleeding and absorption of blood and wound secretions, it protects the damaged tissue from the attack of microbes. It protects against viruses and thus reduces the treatment time. Analysis of biofilms morphology is performed by Field emission scanning electron microscopy (FE-SEM), phases in biofilms were analyzed by X-ray diffraction (XRD) analysis, chemical bonds, and functional groups are analyzed by Fourier transform infrared (FTIR) spectroscopy, and mechanical tests are performed to evaluate the strength of the samples. The thermogravimetric analysis (TGA) is applied to estimate the thermal stability of the biopolymer films with various percentages of CuO/SiO2 nanoparticles. Also, antibacterial test, bioactivity of the biofilms, the percentage of swelling ratio, and porosity of the samples were examined by immersing the samples in simulated body fluid (SBF) and Phosphate-buffered saline (PBS) for 14 days in vitro. The composite makeup of the TA/PVA sample, comprising 15 wt % CuO/SiO2 and containing 15 wt% of nanoparticles, exhibited superior heat resistance compared to other samples by an increase of 50 °C. This improvement can be attributed to the nanoparticles reaching their saturation point. The swelling ratio was assessed in both SBF and PBS, and in both instances, the sample increased by up to 10 wt% before decreasing, indicating the saturation of the nanoparticles.