Design, Development and Invivo Pharmacokinetic Evaluation of Cardiovascular Drug Loaded Nano Structured Lipid Carrier System

Abstract

The Present investigation is to design, develop and optimize the Nanostructured Lipid Carrier (NLC) containing cardiovascular drug by various independent variables like formulation variables (concentration of phospholipids, surfactants, co-surfactant) and process variables (homogenization time and ultrasonication time). The objective of this research is to choose the suitable and reproducible technique for the formulation of NLC based on dependent variables like Particle Size (PS) in nm, Polydispersity Index (PDI), Zeta potential (ZP) in mV and Entrapment efficiency (EE%) by applying Box Behnken design utilizing - Multiple linear regression method (Design expert 9 software, stat-ease, Inc. USA) and another objective is to prove the enhancement of bioavailability of drug in developed NLC. Comparative invivo pharmacokinetic studies was carried out to determine AUC, Cmax, Tmax , Ke, Vd between drug loaded NLC and commercially available dosage form to prove the enhancement of bioavailability in NLC. From the results obtained, it was concluded that hot homogenization was an optimized technique for the preparation of NLC containing carvedilol and NLC shows better bioavailability when compare to commercial formulation. This data was related to the research project sanctioned by Department of science and technology (DST) entitled “Development of Lipid Nanoparticles Enriched Hydrogels for Transdermal Drug Delivery: Formulation, In vitro Characterization and Pharmacodynamic studies in Animals”