Design, Characterization, and Antimicrobial Evaluation of Copper Nanoparticles Utilizing Tamarixinin a Ellagitannin from Galls of Tamarix aphylla.

Mohamed A A Orabi,Hamad S Alyami,El-Shaymaa Abdel-Sattar,Javed Ahmad,Omaish Salman Alqahtani,Tsutomu Hatano,Ehab I Taha,Fakhria A Al-Joufi,Mounir M Salem-Bekhit,Basel A Abdel-Wahab,Ali Mohamed Alshabi

doi:10.3390/ph15020216

Abstract

The application of plant extracts or plant-derived compounds in the green synthesis of metal nanoparticles (NPs) was researched. Determining the exact metabolite implicated in the formation of NPs would necessitate comprehensive investigations. Copper nanoparticles (CuNPs) are gaining a lot of attention because of their unique properties and effectiveness against a wide range of bacteria and fungi, as well as their potential for usage in catalytic, optical, electrical, and microelectronics applications. In the course of this study, we aimed to formulate CuNPs utilizing pure tamarixinin A (TA) ellagitannin isolated from Tamarix aphylla galls. The main particle size of the formed CuNPs was 44 ± 1.7 nm with zeta potential equal to −23.7 mV, which emphasize the stability of the CuNPs. The X-ray diffraction spectroscopy showed a typical centered cubic crystalline structure phase of copper. Scanning electron microscopy images were found to be relatively spherical and homogeneous in shape. The antimicrobial properties of TA, as well as its mediated CuNPs, have been evaluated through well diffusion assays against four bacterial, Bacillus subtilis NCTC 10400, Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 27853, and two fungal, Candida albicans and Aspergillus flavus, strains. The distinctive antimicrobial activities were noted against the fungal strains and the Gram-negative bacterial strains P. aeruginosa ATCC 27853, and E. coli ATCC 25922. In conclusion, CuNPs mediated by TA can be applied for combating a wide range of bacterial and fungal species especially C. albicans, Asp. flavus, and P. aeruginosa in a variety of fields.

Highlights

Nanotechnology is gaining substantial momentum these days owing to remarkably enhanced in vivo fate and subsequently therapeutic efficacy, reduced dose and dosing frequency, and significantly mitigated toxicity profile of incorporated active ingredient in nanoformulation
tamarixinin A (TA) was explored as part of a green chemistry approach for the synthesis of CuNPs
Tamarixinin A ellagitannin was utilized for a facile formulation of CuNPs with small and stable particle sizes

Summary

Introduction

Nanotechnology is gaining substantial momentum these days owing to remarkably enhanced in vivo fate and subsequently therapeutic efficacy, reduced dose and dosing frequency, and significantly mitigated toxicity profile of incorporated active ingredient in nanoformulation. Metal nanoparticles (NPs) including gold, silver, iron, and copper nanoparticles (CuNPs) have been established as potent and efficacious nanoformulation strategies for a diverse variety of indications, microbial infections [1,2,3,4]. Random and/or improper use of antibacterial drugs has resulted in the development of microbial resistance to a range of antimicrobials. Metal NPs have emerged as a possibility for combating these resistant microbes [5]. CuNPs have been widely explored for their antimicrobial potentials in a plethora of research studies, with the results demonstrating that they are effective against a wide range of bacterial and fungal strains [5,6]. The antimicrobial effect of CuNPs could be attributed to their small size, and high surface to volume ratio, which allows them to intermingle with microbial cell membranes appropriately and intimately and exert their microbicide effect [1]

Objectives

Methods

Results

Conclusion