Abstract
Lensless holographic microscope (LHM) is an emerging very promising technology that provides high-quality imaging and analysis of biological samples without utilizing any lens for imaging. Due to its small size and reduced price, LHM can be a very useful tool for the point-of-care diagnosis of diseases, sperm assessment, or microfluidics, among others, not only employed in advanced laboratories but also in poor and/or remote areas. Recently, several LHMs have been reported in the literature. However, complete characterization of their optical parameters remains not much presented yet. Hence, we present a complete analysis of the performance of a compact, reduced cost, and high-resolution LHM. In particular, optical parameters such as lateral and axial resolutions, lateral magnification, and field of view are discussed into detail, comparing the experimental results with the expected theoretical values for different layout configurations. We use high-resolution amplitude and phase test targets and several microbeads to characterize the proposed microscope. This characterization is used to define a balanced and matched setup showing a good compromise between the involved parameters. Finally, such a microscope is utilized for visualization of static, as well as dynamic biosamples.
Highlights
Light microscopy is one of the most employed techniques for analysis in biomedicine, mainly due to its capability to provide images of microscopic specimens using a real-time and non-invasive operation principle
Lensless holographic microscopes were reported in the past years, providing a successful miniaturized solution for, just to cite some examples, global healthcare monitoring, point-of-care diagnosis, and biomedical applications [4]
Practical analysis and characterization of the performance in a Lensless holographic microscope (LHM) is not as common in the literature where only a few references partially cover these issues [6,21]. We present in this contribution a complete analysis of the performance of a compact, reduced cost, and high-resolution lensless holographic microscope based on digital in-line holographic microscopy (DIHM) for biomedical applications
Summary
Light microscopy is one of the most employed techniques for analysis in biomedicine, mainly due to its capability to provide images of microscopic specimens using a real-time and non-invasive operation principle. High-quality imaging is typically provided by optical compound microscopes. Such microscopes are relatively bulky, and often include complicated lens systems which can significantly increase their prices. Those aspects can negatively restrict their use only to advanced laboratories and/or make them neither field-portable nor affordable in practice for developing countries [1]. Lensless microscopes are based on costeffective, light-weight, compact, and portable imaging devices that greatly improve rapid and accurate diagnosis in the field-setting [5,6,7,8,9,10,11,12,13,14,15,16,17,18,19,20,21,22]
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