Abstract
A detailed protocol is described for the design and use of synthetic oligonucleotide probes for screening DNA libraries from Bacillus thuringiensis var. kurstaki ( strain HD191) for copies of the gene (tox) encoding the insecticidal δ-endotoxin. Two homologous tox genes were identified in this organism; one of these was located on a 75-kb plasmid and the other on a second large plasmid or the bacterial chromosome. A tox gene was isolated as a 6.5-kb Hin dIII fragment of B. thuringiensis plasmid DNA.
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