Design and simulation of diatom-based microcantilever immunobiosensor for the early detection of Karnal bunt.

Abstract

Fungal pathogen, Tilletia indica, the cause of Karnal bunt disease in wheat, is severely affecting the yield and grain quality, worldwide. Thus, strict quarantine regulations by most wheat growing countries have to be followed, leading to trade barriers for wheat export. The conventional methods being used for pathogen detection at symptomatic stage requires the germination of Tilletia spores for the processing of samples. Thus, it is time-consuming and expensive. This study proposes a simulated microcantilever-based piezoelectric biosensor for the early detection of T. indica. Four different materials, SiO2, SiC, Si3N4, and Poly Si, were used for the microcantilever design. Microcantilever was coated with siliceous frustules of diatom that provides high surface area and enhanced sensitivity for specific antibody against the antigen, T. indica. Ansys software was used for the simulation analysis. Simulation results showed that microcantilever beam of SiO2 length of 150µm, width of 30µm and thickness 1µm enhanced the sensitivity by two times against the antibody in comparison to normal microcantilever beam. The results concluded that SiO2 with coated diatom is the best material for the microcantilever fabrication, thus, providing an excellent protocol for fabrication of microcantilever-based biosensor which is both cost- and time effective.