Abstract
Human blood is an important medical detection index. With the development in clinical medical detection instruments and detection technology, the requirements for detection accuracy and efficiency have been gradually improved. Fluorescent immunochromatography is a new detection technique. It has the characteristics of high efficiency, convenience, no pollution, and wide detection range. Human blood can be detected quickly using fluorescent immunochromatography. At present, it has received great attention from the field of clinical testing. In this paper, a set of fluorescent immunochromatographic analyzer has been designed. It is mainly based on the principle of fluorescence immunochromatography. A new method of signal analysis and system design for fluorescent immunochromatography analyzer is proposed. By using the improved threshold function denoising algorithm, the quantitative detection of fluorescent immunochromatographic strip is realized. The concentration of pathogenic factors (cancer cells) in human serum can be measured conveniently and accurately. The system integrates many peripheral modules, including fluorescence signal acquisition, fluorescence signal processing, quantitative curve fitting, and test results. In this paper, the quantitative detection experiments of the system are carried out in three aspects: linearity, repeatability, and sensitivity. The experimental results show that the linear correlation coefficient is up to 0.9976, and the limit of detection is up to 0.05 ng/ml. The requirements of the system are satisfied. The system performance is good, and the quantitative result is accurate. Therefore, the establishment of a fluorescence analysis system is of great significance.
Highlights
With the improvement in medical standards and technology, testing instruments are constantly improving
Antigens and antibodies are specific and sensitive, so fluorescent immunochromatography has been widely used in the detection of trace substances in clinical specimens [3, 4]. e most common methods are the radioimmunoassays, the enzyme-labeled immunoassay, the chemiluminescence immunoassay, and the colloidal gold immunoassay. ese analytical techniques have played an important role in biology, medicine, and other fields since they came out one after another in the middle of the 20th century
It uses human eyes to identify, especially in the case of weak positive, which is easy to lead to missed detection, so the colloidal gold immunoassay is more suitable for semiquantitative and manual qualitative detection, but it is difficult to meet the requirements of quantitative detection
Summary
With the improvement in medical standards and technology, testing instruments are constantly improving. Quantitative immunoassay is an immunological method for quantitative detection of various physiological and pathological indexes in samples based on the principle of antigen-antibody reaction or supplemented by various marker-tracer techniques and special detection equipment It has the characteristics of high sensitivity and high specificity. Colloidal gold immunochromatography has the following advantages: observe the results directly with the naked eye without any instrument and equipment, rapid detection, good stability, and it has no toxicity to the operator and no pollution to the environment These characteristics are mainly reflected in the qualitative detection of solid-phase immunity, which cannot meet the clinical needs of accurate and quantitative analysis. If cancer patients can seek medical treatment in time, the design instrument can be used to predict the pathological changes, so as to meet the needs of hospitals and patients
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