Abstract
Column-switching high performance liquid chromatography (HPLC) is extensively used for the critical analysis of radiolabeled ligands and their metabolites in plasma. However, the lack of streamlined apparatus and consequently varying protocols remain as a challenge among positron emission tomography laboratories. We report here the prototype apparatus and implementation of a fully automated and simplified column-switching procedure to allow for the easy and automated determination of radioligands and their metabolites in up to 5 mL of plasma. The system has been used with conventional UV and coincidence radiation detectors, as well as with a single quadrupole mass spectrometer.
Highlights
Positron emission tomography (PET) radioligands designed to image the central nervous system (CNS) are most commonly labeled with carbon-11 or fluorine-18 which have short half-lives of 20.4 and 109.7 min, respectively
It is very rare for neuro-PET radiotracers to resist metabolism to “troublesome” radiometabolites during the course of a PET scan [1,2]
Hilton and coworkers developed a manual column-switching high performance liquid chromatography (HPLC) system for PET radiotracer analysis where plasma is directly loaded onto a capture column from which the analyte is subsequently eluted onto a reverse-phase analytical column [3]
Summary
Positron emission tomography (PET) radioligands designed to image the central nervous system (CNS) are most commonly labeled with carbon-11 or fluorine-18 which have short half-lives of 20.4 and 109.7 min, respectively. Hilton and coworkers developed a manual column-switching HPLC system for PET radiotracer analysis where plasma is directly loaded onto a capture column from which the analyte is subsequently eluted onto a reverse-phase analytical column [3].
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