Abstract
Background: Newcastle disease virus (NDV) causes a highly contagious respiratory, neurological, or enteric disease in chickens. The fusion (F) protein of virus is involved in mediating fusion of the viral envelope with cellular membranes. Heptad repeat region 1 (HR1) peptide of F protein can be used to vaccinate, immunological diagnosis and treat infected avian. As HR1 & HR2 peptide with out of equimolar concentration contains inhibitory effects on NDV entry (fusion), so they can be used to design and produce anti-NDV drugs. Methods: After obtaining virus from poultry we extracted NDV (NR43) RNA using RNX kit, performed RT-PCR technique, and then HR1 cDNA was cloned into pET32a(+) expression vector and transformed into E.coli Bl21(DE3) bacteria by using heat shock. The expression of the recombinant HR1 gene was induced at 30?C temperature using IPTG in 1mM concentration. Results and conclusion: HR1 amino acid and nucleotide sequence was aligned usin g Blast software and deposited at GenBank (AY678224). The achieved peptide was analyzed by using SDS-PAGE and Western-Blotting techniques. Finally, our results clearly demonstrated that the gene can be cloned and expressed in vitro in high dosage and the HR1 peptide can be used as a potentially as a virus fusion inhibitor.
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