Abstract
This study describes the preparation of nanoprotein particles having lignin peroxidase (LiP) using a photosensitive microemulsion polymerization technique. The protein-based nano block polymer was synthesized by cross-linking of ligninase enzyme with ruthenium-based aminoacid monomers. This type polymerization process brought stability in different reaction conditions, reusability and functionality to the protein-based nano block polymer system when compared the traditional methods. After characterization of the prepared LiP copolymer nanoparticles, enzymatic activity studies of the nanoenzymes were carried out using tetramethylbenzidine (TMB) as the substrate. The parameters such as pH, temperature and initial enzyme concentration that affect the activity, were investigated by using prepared nanoLip particles and compared to free LiP. The reusability of the nano-LiP particles was also investigated and the obtained results showed that the nano-LiP particles exhibited admirable potential as a reusable catalyst.
Highlights
Lignin peroxidase (LiP, EC 1.11.1.14) is a heme-containing glycoprotein that has ability to catalyzeH2 O2 dependent-oxidative depolymerization of lignin [1,2]
The maximum hand, immobilized lignin peroxidase (LiP) activity significantly decreased after 40 °C, and the remaining relative activity enzyme activity was obtained at pH 2.5, which is consistent with the results in our study
The relative activity was lower at higher temperatures, and ~70% relative LiP activity was obtained at 60 ̋ C, which is lower than the results obtained in our study
Summary
Lignin peroxidase (LiP, EC 1.11.1.14) is a heme-containing glycoprotein that has ability to catalyze. Enzyme immobilization by chemical conjugation using artificial resins, such as polymers as solid supports, provides an increase in their thermal stability [12,13,14,15]. The protein-based nano block polymer was synthesized by cross-linking of the ligninase enzyme with ruthenium-based amino acid monomers. This type polymerization process brought stability in different reaction conditions, reusability and functionality to the protein-based nano block polymer system when compared the traditional methods. Reusability experiments for the prepared LiP nanoparticles were performed in the last part of the study
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