Abstract
A portable multichannel surface plasmon resonance (SPR) biosensor device is presented in this study. As an optical biosensor device, the core component of its light path is a semi-cylindrical prism, which is used as the coupling unit for the excitation of the SPR phenomena. Based on this prism, a wedge-shaped incident light beam including a continuous angle range (10°) is chosen to replace the commonly-used parallel light beam in traditional SPR devices, in which the incident angle is adjusted by a sophisticated mechanical system. Thus, complicated, cumbersome, and costly mechanical structures can be avoided in this design. Furthermore, the selection of a small and high-stability semiconductor laser and matrix CCD detector as well as a microfluidic system aids in the realization of a miniaturized and multichannel device. Several different samples were used to test the performance of this new device. For ethanol with different concentrations, the sensing response was of good linear relativity with the concentration (Y = 3.17143X + 2.81518, R2 = 0.97661). Mouse IgG and goat anti-mouse IgG were used as biological samples for immunological analysis, and BSA as the control group. Good specific recognition between mouse IgG and goat anti-mouse IgG has been achieved. The detection limit of antibody to antigen coated on the sensing surface was about 25 mg/L without surface modification.
Highlights
Surface plasmon resonance (SPR) biosensors are a widely-used optical sensor, capable of rapidly monitoring the interaction between two kinds of biomolecules in real-time, with no requirement for a label
Owing to its outstanding advantages compared with some other traditional methods such as enzyme-linked immune sorbent assay (ELISA)
The difference of the surface plasmon resonance (SPR) response of 30 min was just about 5% less than that of 60
Summary
Surface plasmon resonance (SPR) biosensors are a widely-used optical sensor, capable of rapidly monitoring the interaction between two kinds of biomolecules in real-time, with no requirement for a label. Owing to its outstanding advantages compared with some other traditional methods such as enzyme-linked immune sorbent assay (ELISA). Some bottlenecks, such as cumbersome and complicated structure as well as high cost, must be overcome. The research and development of miniaturized and high-throughput SPR sensors are attracting wide Sensors attention. An SPR sensing device (Spreeta SPR2000) developed by Texas Instruments (USA) integrated a delicate optical structure in a miniaturized system [12].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
