Abstract
Ethanol production from sugarcane bagasse pith hydrolysate by thermotolerant yeast Kluyveromyces sp. IIPE453 was analyzed using response surface methodology. Variables such as Substrate Concentration, pH, fermentation time and Na2HPO4 concentration were found to influence ethanol production significantly. In a batch fermentation, optimization of key process variables resulted in maximum ethanol concentration of 17.44 g/L which was 88% of the theoretical with specific productivity of 0.36 g/L/h.
Highlights
The global scenario demonstrates that lion share of research in past three decades have been focused on technological know-how development for bioethanol since its emergence as a potential fuel additive
Materials Bagasse pith sample was generated in a depither unit with 100 mesh size at Central Pulp and Paper Research Institute (CPPRI), Saharanpur, Uttar Pradesh and used at CSIR-IIP, Dehradun for hydrolysis and saccharification
sugarcane bagasse (SCB) pith was treated with steam and acid (8% w/w H2SO4) in a solid vs. liquid ratio of 1:10 at 120°C. for 90 minutes to extract pentose rich fraction (20 g/L) which was used as carbon source for cell biomass generation
Summary
The global scenario demonstrates that lion share of research in past three decades have been focused on technological know-how development for bioethanol since its emergence as a potential fuel additive. All the key challenges on energy and economic front have already been pinpointed in various forums as sole restrictors for commercialization of lignocellulosic bioethanol technology (Cardona et al 2010). A non-molasses feedstock was to be brought into reality to meet excess ethanol demand for 5-10% compulsory blending. Biomass being a cheap and renewable raw material with abundant availability (Saxena et al 2009; Kumar et al 2009a; Cheng et al 2008), has been considered as an excellent feedstock for bioethanol production due to its high holocellulosic content. Conversion of lignocellulosic sugar hydrolysate into ethanol requires many other micro
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