Abstract
In this study, a novel multi-targeted magnetic nanoparticles (MTMNPs) are synthesized to investigate their potential application in DNA protection and targeted gene delivery to breast cancer cells. In the present study MTMNPs was prepared from polyethyleneimine coated Fe3O4 nanoparticles, polyethyleneimine-polylactic acid-polyethylene glycol-folic acid copolymer (PEI-PLA-PEG-FA) and DNA using double-emulsion solvent evaporation method. The EPPT peptide (Glu-Pro-Pro-Thr) is also incorporated into the MTMNPs as a targeting agent for overexpressed MUC-1 receptors on breast cancer cells. The DNA release ratio from the MTMNPs nanoparticles were significantly decreased with increasing the pH value and decreasing the temperature. The capability of the MTMNPs nanoparticles to protect the DNA from nuclease digestion and ultrasound damage were confirmed by gel electrophoresis assay. In vitro results demonstrated a novel MFMNPs nanoparticle remarkably reduced PEI-associated toxicity on MCF-7 cells, compared to PEI/DNA complex. Moreover, the MFMNPs improved the efficiency of gene delivery in 10% serum medium by more than 2.98-fold, compared to PEI/DNA complex.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
