Abstract

Avulsed teeth that are replanted dried are more prone to loss. Recent tissue engineering studies focus on fabrication of various cell delivery systems to improve the overall prognosis of such teeth. To evaluate this new cell transplant method, we initially aimed at designing of PRF scaffold and determining BMMSCs viability and function on the fabricated scaffold. To test this concept in-vitro, human BMMSCs were isolated and characterized by cell surface marker, and their potential on osteogenic/adipogenic differentiation. The biological effects of PRF scaffold on human BMMSCs were then investigated by cell proliferation assay. The data were quantified for statistical analysis. It was found that PRF significantly induced BMMSCs proliferation throughout the incubation period due to its growth factor secretion. Results of MTT assay showed an increasing trend during the testing period from day 1 to day 7. The result of scanning electron microscopy showed that BMMSCs could tightly adhere to fibrin scaffold just shortly after seeding. These data suggest that the BMMSCs/PRF construct has the potential to improve the clinical prognosis of replanted avulsed teeth in future. Additional studies are needed to be performed before its clinical use.

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