Abstract
Based on the size of particles, a microfluidic chip integrating micro particles capture, controlled release and counting analysis was designed and fabricated in this paper. The chip is composed of a polydimethylsiloxane (PDMS) cover sheet and a PDMS substrate. The PDMS substrate is made of a sample inlet, microfluidic channels, a micropillar array, a three-dimensional (3D) focusing channel, and a sample outlet. The chip was fabricated by the multistep SU-8 lithography and PDMS molding method in this study. The micropillar array and channels in the chip can be molded in one step and can be replicated multiple times, which reduces the production cost and increases the practicability of the chip. Using a homemade electromagnetic drive device, the detection function of the chip was tested using a deionized water solution containing 22 μm polyethylene particles. The results showed that under the action of electromagnetic force, the chip enriched polyethylene particles; when the electromagnetic force disappeared, the enriched polyethylene particles were released by injecting buffer solution, and it was looked at as new sample solution. The flow rate of the sample solution and the sheath flow solution (deionized water) was injected into the three-dimensional focusing channel at a flow rate ratio of 1:4, and the polyethylene particles sample solution was focused, which could be used for the counting and analysis of polyethylene particles. The work of this paper can provide a reference for the subsequent detection of circulating tumor cells (CTCs).
Highlights
The work of this paper can provide a reference for the subsequent detection of circulating tumor cells (CTCs)
5, the microfluidic chip not subject to external force whe power with power, the sheet isissubjected to electromagnetic power isis off, off,while whileAs with power, thecover cover sheet subjected toisexternal external electromagnetic power is off,deformed while with contact power, the top cover the sheet is subjected to external electromag pressure pressureand andisiselastically elastically deformedto to contactthe the top of of the micropillar micropillar array
The sample injection rate of the microinjection pump was adjusted to 100 μL/min to slowly inject the sample solution into the microfluidic chip
Summary
With the continuous advancement of microfluidic chip technology, they have become a commonly used technology for precision diagnosis as part of precision medicine [2,3,4]
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