Abstract
Over the past several years, the demand for high quality agricultural products has been remarkably increased. Thus, it is important to use non-destructive methods for product quality monitoring. LED-induced fluorescence spectroscopy has proved its potential for nondestructive detection of some defects in agricultural products, such as tissue browning and bruising. Due to such defects, changes in the polyphenol and chlorophyll contents occur which can be considered as the visible marks of decreasing fruit quality. In the present work, a fluorescence spectrometer (spectrofluorometer) controlled by LabVIEW software was designed and developed. In this spectrometer, a consumer-grade webcam was used as an imaging sensor. The spectrometer was able to measure the fluorescence spectra directly from the fruit and vegetable surface in the desired regions. To do so, the spectrometer was equipped with a suitable fiber-optic probe. The hardware solution was based on data acquisition working on the USB platform and controlled by the application running on the PC. In this system, light emitting diodes with different wavelengths were used as the excitation sources for inducing fluorescence spectra of some famous fruits and vegetables.
Highlights
Nowadays, spectral measuring methods such as fluorescence, transmission, remission and diffuse reflection have been investigated for fruit quality control applications [1]
A Laser-induced fluorescence (LIF) spectroscopy system consists of two main parts: a sensitive spectrometer and a source for excitation
Materials and methods In this study a prism-based spectrometer controlled by LabVIEW was developed in order to acquire the spectra in the interval of 571 to 1149 nm
Summary
Spectral measuring methods such as fluorescence, transmission, remission and diffuse reflection have been investigated for fruit quality control applications [1]. Fluorescence spectroscopy has proved its potential for various applications in agriculture, such as detection of tissue browning and bruising [2,3,4] and freshness control [5]. It can be used to monitor water stress in plants [6] Due to such defects, changes in molecular structure of the chlorophyll of products occur which can be considered as visible marks of decreasing fruit quality. Plants show a fluorescence emission in the visible spectral range that is excited by UV radiation and partially by visible light [1].
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