Design and characterization of Poly(glycerol sebacate)/Poly(3-hydroxybutyrate)/bioglass/curcumin nanocomposite scaffold for wound healing application

Abstract

Various applications have been developed for biopolymers, such as scaffolds for tissue engineering. Nanocomposite materials are considered promising for wound healing applications in many unique fields. New nanocomposite scaffold biopolymers were synthesized through the salt leaching technique. Curcumin and bioglass nanoparticles as antibacterial agents were added to Poly(glycerol sebacate)/Poly(3-hydroxybutyrate) nanocomposite scaffolds with different concentrations. Several properties were explored, including morphology, physicomechanical properties, contact angles, antibacterial efficacy, and in vitro studies. The morphology of nanocomposite scaffolds was characterized using SEM and EDX. Additionally, nanocomposite scaffolds Poly(glycerol sebacate)/Poly(3-hydroxybutyrate) showed a water contact angle of 79.8°. The hydrophilicity and water vapor transition rate significantly improved by adding bioglass nanoparticles which were 55° and 2182 g m−2 day−1 for Poly(glycerol sebacate)/Poly(3-hydroxybutyrate)/5 %Bioglass/3 %Curcumin. Samples containing 3 wt% Curcumin had the highest swelling ratio (347 ± 12 %) and the lowest water contact angle. Furthermore, NIH/3T3 fibroblast cells showed significant attachment and viability in in-vitro biocompatibility tests. Bioglass and Curcumin inhibited bacterial growth effectively. Additionally, an in-vitro cell viability, cell attachment, and in-vitro scratch wound healing assay demonstrated that the Poly(glycerol sebacate)/Poly(3-hydroxybutyrates)/5 % Bioglass/3 % Curcumin nanocomposite scaffold could promote wound healing.