Abstract
One of the simpler methods available for detecting the induction of aneuploidy in Drosophila involves the exposure to a suspected mutagen of females homozygous for a readily visible sex-linked recessive mutant allele. The treated females are mated to wild-type males, and the F 1 flies are scored for exceptional progeny (mutant ♀♀ and wild-type ♂♂). The exceptional progeny result from nondisjunction and/or chromosome loss of the X-chromosomes during oogenesis. A mathematical model is presented that describes the “fate” of primary oocytes and which allows one to derive separate estimates of the rates of nondisjunction and chromosome loss during oogenesis. Chromosome loss in this model is defined as the production of nullo-X eggs by any means other than nondisjunction. The model allows for differential viabilities among F 1 genotypes and also allows for the numbers of functional X-bearing and Y-bearing sperm from the male parents to differ from a 1:1 ratio. Statistical procedures are presented that enable one to compare experimental and control groups for rates of nondisjunction and chromosome loss. Interestingly, the spontaneous rate of nondisjunction of X-chromosomes during oogenesis is found to be several times that of chromosome loss.
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