Design and analysis of a CRISPR gene editing strategy in a sheep model variant of GM1 gangliosidosis

Abstract

A new mixed metal borate LiBa1.5[B5O8(OH)3] (1) has been hydrothermally synthesized and structurally characterized by FT-IR spectroscopy, powder X-ray diffraction, single crystal X-ray diffraction, and thermogravimetric analysis, respectively. 1 crystallizes in the monoclinic system, space group C2/c, a = 11.2839(7) Å, b = 7.3974(4) Å, c = 20.7151(13) Å, β = 103.392(6)°, V = 1682.10(17) Å3 and Z = 8. The structure consists of infinite one-dimensional (1-D) borate chains constructed from B5O9(OH)36 − cluster units. These BO chains are further linked by the Ba2 + and Li+ cations to form a 3-D framework.