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Abstract
Drying of seeds at certain stages prior to maturation, i.e. premature desiccation, will terminate synthetic events unique to development, for example, storage protein synthesis, and initiate processes associated with germination. In this study we have investigated the role of desiccation in the expression of a storage protein gene, β-phaseolin, to determine if such a developmentally-regulated gene remains sensitive to drying when controlled by a promoter that has no known sensitivity to this treatment. We compared, in transgenic tobacco seeds, the effects of maturation and premature drying on the expression of a full β-phaseolin gene, and β-phaseolin genes driven by a cauliflower mosaic virus 35S promoter with or without an alfalfa mosaic virus (AMV) 5' untranslated leader sequence
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