Abstract
Desorption electrospray ionization may be used as a fast and convenient method for analysis and identification of lipids in the cell culture. Oxidative stress, which usually involves changes in lipids, was used as a model of pathology to show the utility of this analysis methodology. This paper addresses the surface preparation of cell culture slides, induction of oxidative stress, and cell monolayer culture preparation as well as optimization of the analysis. Advantages and drawbacks of the method were also discussed.
Highlights
Desorption electrospray ionization (DESI) is a mass spectrometry imaging (MSI) method used in the biological sciences for surface analysis
This paper addresses the surface preparation of cell culture slides, induction of oxidative stress, and cell monolayer culture preparation as well as optimization of the analysis
DESI offered a rapid and convenient way of sample measurement/imaging compared to other mass spectrometry imaging techniques based on MALDI and SIMS ion sources that require high vacuum inside the source
Summary
Desorption electrospray ionization (DESI) is a mass spectrometry imaging (MSI) method used in the biological sciences for surface analysis. DESI is a fast and convenient tool for lipid analysis in biological samples (Eberlin et al 2011). Oxidative stress is a pathological state of the cell/ organism where the amount of oxygen radicals increases but cannot be counterbalanced by the internal antioxidant defense system, or when the level of oxidative radicals remains at a physiological state, but the defense system is impaired. This may lead to the oxidative damage of proteins, lipids, and DNA, which usually leads to apoptosis (Feeney et al 2008). Lipids are especially sensitive to this pathophysiology and undergo peroxidation, which results in a number of highly-reactive species that can modify other proteins and lipids, yielding aldehydes and polymerized carbonyl compounds
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