Desflurane anesthesia shifts the circadian rhythm phase depending on the time of day of anesthesia

Ryo Imai,Hiroshi Makino,Takasumi Katoh,Tadayoshi Kurita,Kazuya Hokamura,Kazuo Umemura,Yoshiki Nakajima,Tetsuro Kimura

doi:10.1038/s41598-020-75434-6

Ryo Imai, Hiroshi Makino + Show 6 more

Open Access

https://doi.org/10.1038/s41598-020-75434-6

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Journal: Scientific Reports	Publication Date: Oct 26, 2020
Citations: 14	License type: open-access

Affiliation: Hamamatsu University School of Medicine

Abstract

Desflurane is one of the most frequently used inhalational anesthetics in clinical practice. A circadian rhythm phase-shift after general anesthesia with sevoflurane or isoflurane has been reported in mice, but few studies have reported this effect with desflurane. In the present study, we examined the rest/activity rhythm of mice by counting the number of running wheel rotations, and we found that desflurane anesthesia caused a phase shift in the circadian rhythm that was dependent on the time of day of anesthesia. We also found that desflurane anesthesia altered the relative mRNA expression of four major clock genes (Per2, Bmal, Clock, and Cry1) in the suprachiasmatic nucleus (SCN). These results are important for elucidating the effects of desflurane on the SCN, which is the master clock for the mammalian circadian rhythm. Further studies on the relationship between anesthesia and circadian rhythm may lead to the prevention and treatment of postoperative complications related to circadian rhythms.

Highlights

Desflurane is one of the most frequently used inhalational anesthetics in clinical practice
Entrainment of the circadian clock in mammals mainly depends on light stimuli, which are received by intrinsically photosensitive retinal ganglion cells and inputted into the suprachiasmatic nucleus (SCN) via the retinohypothalamic tract (RHT)[13]
The largest phase shift was observed in the ZT12 subgroup of the anesthesia group, and there was a significant difference between the anesthesia and non-anesthesia groups (− 1.69 h vs. − 0.58 h, p = 0.005)

Summary

Introduction

Desflurane is one of the most frequently used inhalational anesthetics in clinical practice. Previous reports suggested that the effect of sevoflurane and isoflurane anesthesia on the circadian rhythm might differ depending on the time of day of a nesthesia[10,11]. We quantitatively evaluated the effect of desflurane anesthesia on the circadian rhythm by monitoring the rest/activity rhythm in mice and examining its dependence on the time of day of anesthesia.

Results

Conclusion