Desferrioxamine reduces susceptibility to lipid peroxidation in rabbit kidneys subjected to ward ischaemia and reperfusion

Abstract

1. 1. Rabbit kidneys were clamped and subjected to warm ischaemia for 60 or 120 min then reperfused with blood for 60 min or for 24 hr. 2. 2. Treated rabbits received desferrioxamine at 15 or 50mg/kg i.v. 15 man before reperfusion. 3. 3. Their kidneys were then removed and assayed for phospholipid Schiff base fluorescence (ex. 360 nm, era. 435 nm), diene and triene conjugates by UV spectrophotometry (240 nm and 268 nm respectively), for superoxide dismutase and for reduced and oxidised glutathione to provide an index of giutathione redox activity. 4. 4. All indices of lipid peroxidation were significantly elevated in untreated rabbits and glutathione redox activity was reduced. 5. 5. Treatment with desferrioxamine however effectively prevented these deviations and in many cases maintained them at the levels in fresh rabbit kidneys. 6. 6. These data provide further evidence that lipid peroxidation occurring during the reperfusion period is superimposed on the damage set up during warm ischaemia and may be preventable by administration of suitable therapeutic agents.