Abstract
Two new species of Parkellus (Jairajpuri et al., 2001) from Vietnam and a population of Parkellus zschokkei (Ahmad and Jairajpuri, 2010; Menzel, 1913) from Ukraine are described, illustrated and their phylogenetic position among the Mononchida is presented. The molecular data (18S and 28S rDNA) are given for the three investigated species – first time for the genus Parkellus. Parkellus hagiangensis sp. nov. is characterized by a medium-sized buccal cavity, posterior position of the dorsal tooth located below the beginning of the pharynx, males having the ventromedian cuticular pores above and below the excretory pore, short spicules with conical proximal part; females with very faint pars refringens vaginae and small teardrop-shaped pieces, short pars distalis vaginae, the presence of small ventromedian vulval papillae. Parkellus tuyenquangensis sp. nov. is characterized by a medium-sized buccal cavity, posterior position of the dorsal tooth located above the beginning of the pharynx, males having the ventromedian cuticular pores above and below the excretory pore, medium-sized spicules with a cylindrical proximal part, very short lateral guiding pieces, females with very strongly sclerotized pars refringens vaginae, medium size teardrop-shaped pieces, short pars distalis vaginae thickened at the junction with pars refringens vaginae. The newly described species are morphologically most similar to P. parkus and P. zschokkei. An identification key to Parkellus species is presented.
Highlights
Buccal cavity. Zullini and Peneva (2006) and Andrássy (2009) contested such a conclusion assuming that the occurrence of the above mentioned feature is not sufficient to make such a distinction
One of them was the transfer of nine species in which the dorsal tooth is located in the posterior half of the buccal cavity from genus Coomansus to the genus Parkellus
He has separation of several genera based on the position of dorsal tooth apex on dorsal metarhabdion: the new genus Supronchus from the genus Iotonchulus, Megaiotonchus from the genus Iotonchus (Iotonchidae), the new genus Pentonchella (Anatonchidae) (Siddiqi, 2015)
Summary
Nematodes were extracted from soil samples using modified Baermann funnel technique (Southey, 1986) They were heat killed, fixed in 4% formalde hyde (for morphological observations) or in a DESS mixture (Yoder et al, 2006) (for molecular analyses), transferred to anhydrous glycerol (Seinhorst, 1962), and mounted on glass slides for microscopic obser vation. All PCR reactions were performed in Veriti 96-Well Thermal Cycler (Applied Biosystems, Foster City, CA, USA) as follows: an initial denaturation step at 94°C for 3 min, followed by 40 cycles at 94°C for 30 sec, 54°C (58°C or 62°C in case of D2A-D3B primer combination) for 30 sec and 72°C for 60–70 sec with a final incubation for 7 min at 72°C. Stabilization of the likelihood and parameters was checked with the program Tracer (Rambaut et al, 2014: v.1,6)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
