Abstract

We describe and illustrate two techniques for enhancing curatorial and processing efficiency as it pertains to parasitic Hymenoptera (Chalcidoidea, Cynipoidea). These techniques were developed in response not only to the massive number of parasitoids that have been acquired through various biodiversity studies, but also the difficulty in mobilizing the human resources to curate this material. The first technique uses small, crystal polystyrene boxes with tight-fitting lids to store dehydrated specimens prior to mounting. Locality information is affixed to the box and specimens are spread in a layer for ease of examination by researchers. Solutions for managing static electricity within the specimen boxes are discussed. The second involves a vacuum pump connected to a funnel with a filtration membrane and flask apparatus to rapidly dehydrate hard-bodied parasitoids (Figitidae) that are not subject to collapse during air-drying.

Highlights

  • Participation in large-scale biodiversity studies and other ecological research projects (Delabie et al 2000; Droege et al 2010; Fisher 2005; LaPolla et al 2007) involving the collection of arthropods with passive collection techniques

  • After amassing a large volume of raw insect residues from biodiversity studies from which parasitic Hymenoptera must be separated, we employ an aqueous technique using mesh colanders, plastic tubs, and an orbital shaker to separate each raw residue into a macro and a micro fraction (Buffington and Gates 2008)

  • We focus on the micro fraction, sorting higher parasitic Hymenoptera taxa into ethanol for distribution to specialists or further processing at the National Museum of Natural History (USNM)

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Summary

Introduction

Participation in large-scale biodiversity studies and other ecological research projects (Delabie et al 2000; Droege et al 2010; Fisher 2005; LaPolla et al 2007) involving the collection of arthropods with passive collection techniques Fraser et al 2008; Noyes 1989; Townes 1962) generates a massive biomass of interest to the conducting researcher, and a large volume of non-target material, or ‘by-catch.’ Both of these fractions must be dealt with in an efficacious manner to ensure maximal preservation of the morphological and genomic information of the specimens (Quicke et al 1999). After amassing a large volume of raw insect residues from biodiversity studies from which parasitic Hymenoptera must be separated, we employ an aqueous technique using mesh colanders, plastic tubs, and an orbital shaker to separate each raw residue into a macro and a micro fraction (Buffington and Gates 2008).

Materials and methods
Discussion
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