Description of Rotylenchus rhomboides n. sp. and a Belgian population of Rotylenchus buxophilus (Tylenchomorpha: Hoplolaimidae).

Huu Tien Nguyen,Wilfrida Decraemer,Phougeishangbam Rolish Singh,Wim Bert,Marjolein Couvreur,Quang Phap Trinh

doi:10.21307/jofnem-2019-023

Huu Tien Nguyen, Wilfrida Decraemer + Show 4 more

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https://doi.org/10.21307/jofnem-2019-023

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Abstract

During a survey in the Botanical garden of Ghent University, a new species Rotylenchus rhomboides n. sp. and a population of Rotylenchus buxophilus were found. Rotylenchus rhomboides n. sp. is characterized by the presence of a rhomboid-like widening of the mid-ridge of lateral field at the level of vulva, a feature previously unknown within the genus. The population of the new species, composed only by females, has a rounded labial region with 4 to 5 annuli, robust stylet (31–37 μm long), short dorsal esophageal gland (9–19 μm) overlap of the intestine, vulva located slightly posterior to mid-body, and hemispherical or rounded tail shape with large phasmids located 3 to 5 annuli anterior to the level of anus. The hierarchical cluster analysis based on morphological features indicated that the new species closely resembles R. corsicus, R. gracilidens, and R. rugatocuticulatus. The DNA analyses of the D2-D3 of 28S rDNA, ITS rDNA, and COI mtDNA sequences of Rotylenchus rhomboides n. sp. show a close relationship with R. buxophilus, R. goodeyi, R. laurentinus, R. pumilus, and R. incultus, all of which can also be differentiated from the new species by morphological features. The combination of morphological, morphometric, and molecular characteristics confirmed the new species and the first report of R. buxophilus on yam (Dioscorea tokoro) in Belgium.

Highlights

The type species Rotylenchus robustus (De man, 1876) was originally described as Tylenchus robustus by De man (1876)
The hierarchical cluster analysis based on morphological features indicated that the new species closely resembles R. corsicus, R. gracilidens, and R. rugatocuticulatus
The DNA analyses of the D2-D3 of 28S rDNA, ITS rDNA, and COI mtDNA sequences of Rotylenchus rhomboides n. sp. show a close relationship with R. buxophilus, R. goodeyi, R. laurentinus, R. pumilus, and R. incultus, all of which can be differentiated from the new species by morphological features

Summary

Introduction

The type species Rotylenchus robustus (De man, 1876) was originally described as Tylenchus robustus by De man (1876). A Belgian population of Rotylenchus buxophilus (Loof and Oostenbrink, 1958) have been reported in Belgium (Steel et al, 2014; Viaene et al, 2017). They are the main cause of yield losses in many agricultural crops such as carrot, olive, orange, mango, soybean, broccoli, cabbage, tomatoes, etc. Is characterized and a population of R. buxophilus is reported for the first time on yam (Dioscorea tokoro Makino) Both species were described based on morphology and morphometrics along with molecular characteristics and phylogeny of the D2-D3 expansion segment of 28S rDNA, ITS rDNA, and COI mtDNA sequences

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