Description of Pseudaphelenchus zhoushanensis n. sp. (Tylenchina: Aphelenchoididae) found in the wood of Pinus thunbergii at Zhoushan Islands, Zhejiang Province, China

Abstract

Pseudaphelenchus zhoushanensisn. sp. was isolated from a deadPinus thunbergiiat Changgang Mountain, Zhoushan Islands, Zhejiang Province, China. It is characterised by the small to medium length body, cuticle slightly annulated, presence of three lateral lines, stylet 9.0-10.7 μm with small but conspicuous basal knobs, excretory pore located from same level as the metacorpus to slightly anterior to metacorpus, true bursa surrounding entire tail but inconspicuous, male tail conical with a single mucron, spicule with distinct condylus and rostrum strongly arcuate to a pointed end, female tail conical with annulation, strongly ventrally bent in distal part of tail, with terminus bluntly pointed or finely mucronate. Phylogenetic analyses using sequences of the18S and 28S D2-D3 regions of rDNA confirmed the status ofP. zhoushanensisn. sp. as a new species. Combining the molecular phylogenetic analyses, morphology and biology ofP. zhoushanensisn. sp. andTylaphelenchus jiaaeindicates thatT. jiaaeis a member ofPseudaphelenchusto which it is herein transferred asP. jiaaen. comb. (=T. jiaae).