Abstract
Pseudacrobeles (Pseudacrobeles) curvatus sp. n. was collected from potato fields in Gyeongsangnam-do, South Korea. The new species shares morphological characters typical of the genus Pseudacrobeles, including three lateral incisures that fade posteriorly near the phasmid openings. The new species differs from other Pseudacrobeles species by its smaller body size and a comparatively shorter corpus relative to the isthmus length. In this study, we provide a comparison of morphometrics and diagnostic features of Pseudacrobeles species and molecular sequence data from the D2-D3 regions of the 28S ribosomal DNA (rDNA) and ITS1-5.8S-ITS2 region of rDNA from the new species, which can be used as molecular barcode sequences.
Highlights
Pseudacrobeles (Pseudacrobeles) curvatus sp. n. was collected from potato fields in Gyeongsangnam-do, South Korea
A new species, described as Pseudacrobeles (Pseudacrobeles) curvatus sp. n., was isolated from soil samples collected from potato fields in South Korea
A detailed morphological description based on morphometric analyses including observations from light microscopy (LM) and scanning electron microscopy (SEM) is provided
Summary
Nematode isolation and culture: Soil samples were collected from potato farms in Changwon-si, Gyeongsangnam Province, South Korea. Morphological observations: Individual nematodes were placed in 2 ml water in a 15 ml tube and quickly fixed with the addition of 4 ml of 808C TAF (2% triethanolamine and 7% formaldehyde), processed to dehydrated glycerin following Seinhorst (1959) and mounted in pure glycerin on HS-slides (Shirayama et al, 1993). Sequences of the ITS1-5.8S-ITS2 region and the D2-D3 regions of 28S rDNA were aligned to their homologous gene sequences of some other Pseudacrobeles species available from GenBank, in Clustal X with default options (Thompson et al, 1997). Both ends of the aligned datasets were trimmed before sequence analyses were performed using the Genious 6 program
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