Abstract
BackgroundThe importance of wheat to the world economy, together with progresses in high-throughput next-generation DNA sequencing, have accelerated initiatives of genetic research for wheat improvement. The availability of high density linkage maps is crucial to identify genotype-phenotype associations, but also for anchoring BAC contigs to genetic maps, a strategy followed for sequencing the wheat genome.ResultsHere we report a genetic linkage map in a durum wheat segregating population and the study of mapped DArT markers. The linkage map consists of 126 gSSR, 31 EST-SSR and 351 DArT markers distributed in 24 linkage groups for a total length of 1,272 cM. Through bioinformatic approaches we have analysed 327 DArT clones to reveal their redundancy, syntenic and functional aspects. The DNA sequences of 174 DArT markers were assembled into a non-redundant set of 60 marker clusters. This explained the generation of clusters in very small chromosome regions across genomes. Of these DArT markers, 61 showed highly significant (Expectation < E-10) BLAST similarity to gene sequences in public databases of model species such as Brachypodium and rice. Based on sequence alignments, the analysis revealed a mosaic gene conservation, with 54 and 72 genes present in rice and Brachypodium species, respectively.ConclusionsIn the present manuscript we provide a detailed DArT markers characterization and the basis for future efforts in durum wheat map comparing.
Highlights
The importance of wheat to the world economy, together with progresses in high-throughput next-generation DNA sequencing, have accelerated initiatives of genetic research for wheat improvement
The availability of high density linkage maps is crucial to identify genotype-phenotype associations, and for anchoring BAC contigs to genetic maps, a strategy followed for sequencing the wheat genome, promoted by the International Wheat Genome Sequencing Consortium (IWGSC, http://www.wheatgenome.org/) [1]
Five loci corresponding to five genes were included in the genetic map since they resulted polymorphic between the two parents [21,22,23]
Summary
The importance of wheat to the world economy, together with progresses in high-throughput next-generation DNA sequencing, have accelerated initiatives of genetic research for wheat improvement. The availability of high density linkage maps is crucial to identify genotype-phenotype associations, and for anchoring BAC contigs to genetic maps, a strategy followed for sequencing the wheat genome. Wheat (Triticum spp.) is among the most widely grown crops in the world. It is a polyploid species with a nuclear genome characterized by seven homoeologous chromosome groups (A, B and D genomes). The availability of high density linkage maps is crucial to identify genotype-phenotype associations, and for anchoring BAC contigs to genetic maps, a strategy followed for sequencing the wheat genome, promoted by the International Wheat Genome Sequencing Consortium (IWGSC, http://www.wheatgenome.org/) [1]. The availability of the DArT marker sequences and their abundance in grass genomes changed the role of these markers, proposing them as an optimal tool for comparative genetic mapping or for identification of functional markers
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