Description of Algoriphagus iocasae sp. nov., isolated from deep-sea sediment.

Abstract

In this paper, we report on the identification and species characterization of a novel Gram-staining-negative bacterium, strain S61T, isolated from the deep-sea sediment of the Okinawa Trough. Growth of strain S61T occurred at 4-37 °C (optimum, 25 °C), pH 6.0-10.0 (optimum, pH 7.0) and with 0-8 % (w/v) NaCl (optimum, 2 %). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain S61T belonged to the genus Algoriphagus. The closest phylogenetic neighbours of strain S61T were Algoriphaguslutimaris S1-3T (98.1 %), Algoriphagushalophilus JC2051T (98.1 %), Algoriphaguschungangensis CAU 1002T (98.0 %) andAlgoriphagusaestuarii MDM-1T (97.4 %). The sequence similarities between strain S61T and other close members of the genus were below 97 %. The values of DNA-DNA relatedness between strain S61T and its closest relatives in the genus Algoriphagus were well below 70 %. The genomic DNA G+C content of strain S61T was 40.2 mol%. The major menaquinone was MK-7. The polar lipids contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and two unidentified phospholipids and one unidentified lipid. The major fatty acids were iso-C15 : 0 and summed feature 3 (C16 : 1ω6c and/or C16 : 1ω7c). Phylogenetic, physiological, biochemical, and morphological analyses suggested that this strain represents a novel species of the genus Algoriphagus, for which the name Algoriphagus iocasae sp. nov. is proposed with the type strain S61T (=KCTC 52359T=CCTCC AB 2015446T).