Abstract

In France since 1985, corn salad (Valerianella locusta) production has been increasing by 820 t a year and now reaches nearly 15 000 t. This increase was followed by the outbreak of a new disease in the main production area. In 1991, a pathogenic bacterium was isolated from black and greasy leaf spots. Studies based on phenotypic and genetic characteristics have been carried out to describe this new bacterium first identified as a non-fluorescent Pseudomonas (1). As Phoma valerianellae is a fungus also responsible for black leaf spots on corn salad, a reliable method of diagnosis was needed to identify the disease in routine. Immunofluorescence performed on symptoms and double-diffusion on pure cultures were tested.KeywordsPure CultureReference StrainPathogenic BacteriumLeaf SpotPathogenicity TestThese keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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