Abstract

AbstractHomogenates of cotyledons from immature soybean seeds were fractionated by centrifugation. Cell‐free preparations actively desaturated 1‐14C‐palmitate and 1‐14C‐stearate to produce 9,10‐unsaturated acids. The 9‐desaturase activity was present mainly in supernatant fractions (22,000 and 105,000 xg). These fractions also desaturated oleate to linoleate and elongated C14, C16 and C18 acids. In view of this versatility of desaturase systems in the soybean, including the 9‐desaturase(s) for C14, C16 and C18 saturated acids, there would not seem to be further need to consider a separate plant pathway for biosynthesis of unsaturated fatty acids.

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