Dernières mises au point concernant la conservation des concentrés de plaquettes à 4 °C

Abstract

Summary Under current blood-banking practices, platelet concentrates (PC) can be stored for 5 days from 20 ° to 24 °C. The storage life is essentially limited because storage to 22 °C leads to an increase in the risk of bacterial contamination and to unacceptable lesions of platelets. Therefore, there is considerable interest in developing methods to improve storage conditions. In the past, platelet storage at 4 °C has been extensively studied. The advantages of the refrigerated temperature included an inhibition of the growth of bacterial contaminants and a reduction of metabolic rates which reduce the deleterious effects observed with the storage of platelets at room temperature. Unfortunately, a rapid loss of in vitro functional activity and in vivo viability was observed at 4 °C. This report summarizes storage lesions of PC at 4 °C, and evaluates the effects of a new solution (thrombosol) made up of second messenger effectors. Thrombosol provides a protective effect against in vitro platelet storage lesions and cold-induced activation. Treated platelets stored at 4 °C displayed significantly greater recovery of in vitro activity than did control platelets stored at 22 °C. This is a strong basis that supplementation of platelet concentrates with thrombosol might allow the storage of PC at 4 °C.