Dermal Delivery Enhancement of Natural Anti-Ageing Compounds from Ocimum sanctum Linn. Extract by Nanostructured Lipid Carriers.

Pachabadee Marsup,Suvimol Somwongin,Wantida Chaiyana,Songyot Anuchapreeda,Wei-Chao Lin,Kuan-Han Lee,Shang-Chian Lue

doi:10.3390/pharmaceutics12040309

Pachabadee Marsup, Suvimol Somwongin + Show 5 more

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https://doi.org/10.3390/pharmaceutics12040309

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Abstract

This study aimed to develop nanodelivery systems for enhancing the Ocimum sanctum Linn. extract delivery into the skin. Rosmarinic acid (RA) was used as a marker for the quantitative determination of the extract by high-performance liquid chromatography. Nanostructured lipid carriers (NLC), nanoemulsion, liposome, and niosome, were developed and characterized for internal droplet size, polydispersity index (PDI), and zeta potential using photon correlation spectroscopy. Irritation properties of each formulations were investigated by hen’s egg test on the chorioallantoic membrane. In vitro release, skin permeation, and skin retention are determined. NLC was suggested as the most suitable system since it enhances the dermal delivery of RA with the significant skin retention amount of 27.1 ± 1.8% (p < 0.05). Its internal droplet size, PDI, and zeta potential were 261.0 ± 5.3 nm, 0.216 ± 0.042, and −45.4 ± 2.4 mV, respectively. RA released from NLC with a sustained release pattern with the release amount of 1.29 ± 0.15% after 24 h. NLC induced no irritation and did not permeate through the skin. Therefore, NLC containing O. sanctum extract was an attractive dermal delivery system that was safe and enhanced dermal delivery of RA. It was suggested for further used as topical anti-ageing products.

Highlights

As the proportion of the ageing population continues to increase, the dermatological concerns of the aged grow in medical importance [1]
The topical formulations are expansively used because of the lower price, ease of application, and lack of injury comparing to a medical device treatment
The extract would not be effective unless it could penetrate into viable epidermis or deeper into the dermis

Summary

Introduction

As the proportion of the ageing population continues to increase, the dermatological concerns of the aged grow in medical importance [1]. The topical formulations are expansively used because of the lower price, ease of application, and lack of injury comparing to a medical device treatment. The effectiveness of those formulations is still suspicious as well as the effectiveness of the active ingredients. Natural cosmetic active compounds are currently popular due to the growing concern and caution about the potential harmful effects of synthetic ingredients [2]. Our previous study revealed a potent anti-skin-ageing activity of Ocimum sanctum ethanolic extract, including antioxidation, anti-inflammation, inhibition against hyaluronic acid, and collagen fiber degradation inhibition [3]. O. sanctum ethanolic extract is an attractive cosmetic active ingredient for anti-skin-ageing products. Especially nanodelivery systems, were important to overcome these problems

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