Abstract
Conventional reversed-phase HPLC conditions have been optimized for resolution of a mixture containing prostaglandins PGE 1, PGE 2, PGF 1α, and PGF 2α. Electroactive derivative-forming reagents, such as p-nitrobenzyloxyamine, 2-bromo-2′-nitroacetophenone, and 2,4-dinitrophenylhydrazine have been evaluated for use as precolumn reagents for forming prostaglandin derivatives. The results indicate that detection limits of 120 pg are achievable with amperometric detection. The utility of the procedures developed is illustrated by the detection of prostaglandins in human urine and plasma.
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