Abstract
Simple and precise derivative UV spectrophotometric method by first and second order have been developed and validated for the estimation of metformin hydrochloride in bulk and its tablet formulation. The standard and sample solutions of metformin hydrochloride were prepared in distilled water. Metformin hydrochloride was estimated by first and second order at wavelength 225nm and 252nm respectively. Beer's law was obeyed in the concentration range of 1 to 14μg/ml with coefficient of correlation value 0.9999. These methods were tested and validated for various parameters according to ICH guidelines. The precision expressed as relative standard deviation were 0.6787% for first order and 0.2421% for second order derivative method respectively. The proposed method was successfully applied for the determination of metformin hydrochloride in pharmaceutical formulation. Results of the analysis were validated statistically and were found to be satisfactory. The proposed method is simple, easy to apply, low-cost and require relatively inexpensive instruments.
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