Abstract
The human embryonic stem cell line RCe012-A (RC-8) was derived from a frozen and thawed day 5 embryo cultivated to the blastocyst stage. The embryo was voluntarily donated as unsuitable and surplus to fertility requirements following ethics committee approved informed consent under licence from the UK Human Fertilisation and Embryology Authority. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data is available.
Highlights
The human embryonic stem cell line RCe012-A (RC-8) was derived from a frozen and thawed day 5 embryo cultivated to the blastocyst stage
RCe012-A (RC-8) was derived from a thawed day 5 blastocyst that was surplus to requirement or unsuitable for clinical use
A microsatellite PCR profile has been obtained for the cell line, and Human Leukocyte Antigen (HLA) Class I and II typing is available (Table 2)
Summary
Citation for published version: De Sousa, P, Tye, BJ, Bruce, K, Dand, P, Russell, G, Collins, DM, Greenshields, A, Bradburn, H, Downie, JM, Bateman, M & Courtney, A 2016, 'Derivation of the human embryonic stem cell line RCe012-A (RC-8)', Stem Cell Research, vol 16, no. 2, pp. 489-492. https://doi.org/10.1016/j.scr.2016.02.033 General rights Copyright for the publications made accessible via the Edinburgh Research Explorer is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights.
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