Abstract
The human embryonic stem cell line RCe009-A (RC-5) was derived from a frozen and thawed Day 2 embryo voluntarily donated as unsuitable and surplus to requirement for fertility treatment following informed consent under licence from the UK Human Fertilisation and Embryology Authority. RCe009-A carries the common DF508 mutation on the cystic fibrosis trans-membrane regulator gene associated with the disease cystic fibrosis. The cell line shows normal pluripotency marker expression and differentiation to the three germ layers in vitro. It has a normal 46XX female karyotype and microsatellite PCR identity, HLA and blood group typing data are available.
Highlights
RCe009-A carries the common DF508 mutation on the cystic fibrosis trans-membrane regulator gene associated with the disease cystic fibrosis
The cell line was derived by whole embryo outgrowth on mitotically inactivated human fibroblast (HDF) feeder cells using human dermal fibroblasts (HDFs) conditioned medium and expanded under feeder free conditions
By Day 8 of development, or when spontaneous hatching occurred, embryos were placed in derivation conditions consisting of mitotically inactivated neonatal human dermal fibroblasts (HDFs) (ThermoFisher Scientific (Cascade Biologics), Paisley, UK) on tissue culture plastic precoated with 2 μg/cm2 human laminin (Sigma Aldrich, Dorset, UK) as per manufacturer's recommendation
Summary
Citation for published version: De Sousa, P, Tye, B, Bruce, K, Dand, P, Russell, G, Collins, DM, Gardner, J, Downie, JM, Bateman, M & Courtney, A 2016, 'Derivation of the human embryonic stem cell line RCe009-A (RC-5)', Stem Cell Research, vol 16, no. 2, pp. 418-422. https://doi.org/10.1016/j.scr.2016.02.030 General rights Copyright for the publications made accessible via the Edinburgh Research Explorer is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights.
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