Derivation of substrate specific non starch polysaccharide enzymes cocktail for commercial layer diets containing alternate protein supplements

Abstract

The study was conducted in two phases. In phase I, a com-soybean meal based diet, was prepared as a control and another two diets (APS1 and APS2) containing alternate protein supplements were prepared by replacing 44 and 87% of SBM in control diet with cotton seed meal (CSM), rapeseed meal (RSM) and guar meal (GM) at three levels i.e., 5, 2.5 and 3% respectively, in APS1 and 10, 5 and 6%, respectively in APS2. The suitable NSP enzymes namely xylanase (X), mannanase (M), cellulase (C), P-glucanase (G) and pectinase (P) for in vitro digestion assay was selected based on soluble NSP sugars in CSM, RSM and GM. Among various concentrations and combinations tested X-700, M-1000, C-100, G-1400 and P-2000 IU/kg for corn/SBM; X-2100, M-1000, C-3000, G-2800 and P-7000 IU/kg for APS1 and X-40000, M-6000, C-1900, G-10000 and P-10000 IU/kg for APS2 released highest (P<0.001) sugar concentration. In phase II, in vivo study was conducted to assess NSP enzymes supplementation on nutrient utilization and energy efficiency in cockerels fed corn/SBM, APS1 and APS2. Significantly highest (P<0.05) organic matter (OM), crude protein (CP), crude fibre (CF) utilization and energy efficiency was observed in birds fed corn-SBM, APS1 and APS2 diets supplemented with respective NSP enzymes, which released maximum sugars in vitro. Thus present study indicates that NSP enzymes selected based on NSP sugars in feed ingredients and their combination with maximum sugar release in vitro could be taken as a criterion for deriving suitable NSP enzyme cocktail for commercial layer diets containing alternate protein supplements.