Abstract
Induced pluripotent stem cells (iPSCs) are powerful tools for basic and translational research, as well as regenerative medicine. In routine human in vitro fertilization (IVF) practices, cumulus cells (CCs) are discarded, representing a potential source of biological materials for regenerative medicine. In this study, we derived patient-specific iPSCs using CCs from human infertility clinics for the first time. The human cumulus cell derived iPSCs (hc-iPSCs) were characterized for growth, karyotype, expression of pluripotency genes, and were subjected to embryoid bodies (EBs) and teratoma assays to evaluate their differentiation capacity. Hc-iPSCs display typical iPSC characteristics, and are capable of differentiating into all germ layers in vitro and in vivo. We further show that putative primordial germ cell like cells (PGCLCs) can be derived using hc-iPSCs. Our data demonstrate the feasibility of deriving patient-specific pluripotent stem cells using CCs.
Highlights
The induced pluripotent stem cell technology is one of the most important breakthroughs in regenerative medicine in the past decade [1,2,3]
The aim of this study is to prove that clinically discarded cumulus cells (CCs) can be utilized to derive patient-specific Induced pluripotent stem cells (iPSCs), and once established, to evaluate their differentiation capacity in vitro and in vivo
CCs were obtained from seven patients (Table 1) who had undergone intracytoplasmic sperm injection (ICSI) at Memorial Hospital (MMH) in vitro fertilization (IVF) clinic with patients’ consent
Summary
The induced pluripotent stem cell (iPSC) technology is one of the most important breakthroughs in regenerative medicine in the past decade [1,2,3]. Using this technology, researchers can directly convert somatic cells to pluripotent states, bypassing the need for human embryos and avoiding related ethical, religious and legal concerns that are associated with human embryonic stem cells (ESCs) [4,5,6]. IPSCs can be patient-specific, have the potential to solve the immunological compatibility issues. Patient Specific Pluripotent Stem Cells from Human CCs IPSCs can be derived using versatile methods including both integrating
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