Abstract
Publisher Summary This chapter presents stepwise methodology using different conditions and techniques to promote the derivation and maintenance of human embryonic stem cells (hESCs), including conditions that support the culture of human blastocysts and the basic characterization of newly established lines. Researchers need to be well acquainted with the legal and ethical standards governing their countries, states, and institutions. Derivation of hESCs requires approval from an authorized institutional review board. The hESCs are derived from the inner cell mass of in vitro fertilized embryos, which are donated after consent from both maternal and paternal gametes. Derivation and subsequent maintenance of hESCs are described using numerous conditions, including a variety of growth substrates and several types of feeder and nonfeeder systems. Recently, progress has been made toward deriving hESCs using chemically defined, xeno-free media, which may expedite further therapeutic approaches.
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