Der Abbau der Epidermis-Proteine der Raupe Galleria mellonella mittels der durch den entomophagen Pilz Beauveria bassiana sezernierten toxischen proteolytischen Enzyme : The Degradation of the Cuticle Proteins of Greater Wax Moth Larvae ( Galleria mellonella) by Toxic Proteolytic Enzymes, Secreted by the Entomophagous Fungus Beauveria bassiana

Abstract

Summary Cultivation media of the entomophagous fungus Beauveria bassiana, containing two proteolytic enzymes, were applied to purified cuticles of Galleria mellonella larvae. As seen from the results, the proteolytic enzymes (with the pH. optima 5 and 8) acted as toxins to Galleria mellonella larvae, on the one hand, and on the other hand, as enzymes which degrade the cuticle proteins. This was demonstrated both qualitatively by staining in situ the individual cuticle components and quantitatively by the decrease of total nitrogen in the substrate in dependence on time, as well as by the increase in amino nitrogen in the reaction medium.