Abstract
The annual rate of CH 4 release and potential CH 4 production has recently been studied in the Salmisuo fen in eastern Finland but the microbiota responsible for the CH 4 production has not been examined. The diversity of the methane producing Archaea was analysed, at different depths, in the most representative microsite ( Eriophorum lawn) of the fen. Methanogen populations were studied using primers amplifying a region of the methyl-coenzyme M reductase gene. PCR products were analysed by denaturing gradient gel electrophoresis and restriction fragment length polymorphism (RFLP) analysis of clone libraries. A representative of each RFLP group was sequenced. The study revealed a change of the methanogen populations with depth. Sequences from the upper layers of the fen grouped in a novel ‘Fen cluster’ and were related to Methanomicrobiales. Sequences retrieved from the deeper layers of the fen were related to Methanosarcinales via the Rice Cluster-I.
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