Abstract
A new technique that can increase the depth of field (DOF) for in vivo tissue imaging is presented. Extended DOF for the imaging of strongly scattering media is realized through combining laser speckle contrast imaging (LSCI) with light field (LF) microscopy. To the best of our knowledge, it is for the first time to combine these two imaging techniques into one system to provide enhanced functionality. The entire imaging process requires only one single detection with no mechanical adjustment involved. The feasibility and capability of the proposed technique is experimentally demonstrated in volumetric flow speed measurement.
Highlights
Laser speckle contrast imaging (LSCI) is a wide field of view, non-scanning optical imaging scheme capable of measuring relative blood flow speeds
We propose a LSCI system with light field (LF) microscope approach to extend the depth of field for the first time
A preliminary attempt has been made to measure the flow velocities at different depths with just one single detection. These results show that imaging in strongly scattering media with depth of field (DOF) extension can be realized with the combination of LF and LSCI
Summary
Laser speckle contrast imaging (LSCI) is a wide field of view, non-scanning optical imaging scheme capable of measuring relative blood flow speeds. For an object contains several individual moving particles, such as red blood cells, the spackle pattern is dynamic and changes in time This will cause a blurring of the speckle and lead to a reduction in the local speckle contrast [3], [4]. X. Ma et al.: Depth of Field Extension in Laser Speckle Contrast Imaging be adopted to increase the DOF: sweeping the camera’s focal length or narrowing the lens aperture. One can create a virtual renders of the light field at any position by resampling and interpolating the light rays, which bring LF imaging some unique functions, such as: refocusing the image, gathering the depth information and extending the DOF [14]. A preliminary attempt has been made to measure the flow velocities at different depths with just one single detection These results show that imaging in strongly scattering media.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
