Abstract
It has been reported that the deoxythymidylate-deoxyadenylate-rich satellite DNA from the land crab is lost into the phenol phase during phenol extraction of tissues from that organism. Others report that alternating poly deoxyadenylate-deoxythymidylate is lost into the phenol phase during deproteinization. The reasons for this selective loss were investigated, and it was shown that the high NaCl concentrations used during extraction are responsible. A phenol extraction method that gives quantitative recovery of alternating poly deoxyadenylate-deoxythymidylate and other enzymically synthesized polymers is described.
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